Interplay of Quorum-Sensing Molecules and Yeast Growth Dynamics: Insights from Strain-Specific Responses
Presenter Major
Biology Pre-Professional/Graduate Studies
Presentation Type
Oral
Location
Hays Theatre, Wilbur Arts Building
Start Date
26-4-2024 11:45 AM
End Date
26-4-2024 11:50 AM
Description (Abstract)
This study investigates the impact of quorum-sensing molecules (QSMs) on the growth of five yeast strains, including Candida albicans, two novel strains from blueberry surfaces (NPBB1 and NPBB2), and two laboratory standard strains (L5487 and L5489). QSMs, known for their potential as anti-microbial agents due to their ability to disrupt microbial social behaviors, are particularly relevant in combating biofilm-associated infections, where microbial communities exhibit heightened resistance to traditional antimicrobial treatments. Optimal pH and temperature conditions for yeast growth were first established for each strain. Subsequently, three QSMs—farnesol, 2-dodecanol, and 2-phenylethanol—were introduced at specified concentrations into liquid cultures of the yeast strains. Following a two-day incubation period, yeast cell quantification revealed distinct effects of the QSMs on planktonic growth across the various strains. Notably, the results demonstrate strain-specific responses to the QSMs, suggesting a nuanced interplay between QSMs and yeast growth dynamics. These findings underscore the significant role of QSMs in mediating yeast communication and social behaviors. By elucidating the differential effects of QSMs on yeast strains, this study contributes to a deeper understanding of microbial interactions and may inform the development of novel antimicrobial strategies targeting quorum-sensing pathways.
Keywords
quorum-sensing molecules, quorum-sensing, yeast, microbial interactions
Related Pillar(s)
Study
Interplay of Quorum-Sensing Molecules and Yeast Growth Dynamics: Insights from Strain-Specific Responses
Hays Theatre, Wilbur Arts Building
This study investigates the impact of quorum-sensing molecules (QSMs) on the growth of five yeast strains, including Candida albicans, two novel strains from blueberry surfaces (NPBB1 and NPBB2), and two laboratory standard strains (L5487 and L5489). QSMs, known for their potential as anti-microbial agents due to their ability to disrupt microbial social behaviors, are particularly relevant in combating biofilm-associated infections, where microbial communities exhibit heightened resistance to traditional antimicrobial treatments. Optimal pH and temperature conditions for yeast growth were first established for each strain. Subsequently, three QSMs—farnesol, 2-dodecanol, and 2-phenylethanol—were introduced at specified concentrations into liquid cultures of the yeast strains. Following a two-day incubation period, yeast cell quantification revealed distinct effects of the QSMs on planktonic growth across the various strains. Notably, the results demonstrate strain-specific responses to the QSMs, suggesting a nuanced interplay between QSMs and yeast growth dynamics. These findings underscore the significant role of QSMs in mediating yeast communication and social behaviors. By elucidating the differential effects of QSMs on yeast strains, this study contributes to a deeper understanding of microbial interactions and may inform the development of novel antimicrobial strategies targeting quorum-sensing pathways.
