Journal Title or Book Title
Mesenchymal stem cells (MSC) are the progenitor cells to connective tissue cells, epithelial cells, and smooth muscle cells. These cells are currently being investigated as a cellular source for tissue regeneration and repair. The systemic and local tissue environment may have significant influence on the success of such efforts. We hypothesized that elevated glucose and exposure to stress hormones would influence the proliferation of MSC. MSC from the bone marrow of Wistar-Kyoto (WKY) rats were grown under both low glucose (5 mM) and high glucose (20 nM) conditions in the presence and absence of the synthetic glucocorticoid, dexamethasone, and adrenocorticotropin hormone (ACTH). Relative cell density was used to determine the rate of proliferation and was measured using methylene blue staining. Changes in cell density from initial plating were recorded at various stages of culture. Under low-glucose conditions, stress hormones reduced MSC proliferation and these changes were enhanced when cells were exposed to high glucose conditions. These data indicate that stress and elevated glucose can have a significant effect on the ability of MSC to proliferate and may prove to reduce their efficacy during tissue repair.
Evans, Jodi F. Ph.D.; Abrego, Nancy; and Ragolia, Louis, "High Glucose Enhances the Proliferation Effects of Stress Hormones in Mesenchymal Stem Cell Cultures" (2011). Faculty Works: Biology, Chemistry, and Environmental Studies. 15.