Document Type

Peer-Reviewed Article

Publication Date

2017

Journal Title or Book Title

Stem Cells International

Volume

2017

Version

Publisher's PDF

Abstract

Mesenchymal progenitor cell characteristics that can identify progenitor populations with specific functions in immunity are actively being investigated. Progenitors from bone marrow and adipose tissue regulate the macrophage (MΦ) inflammatory response by promoting the switch froman inflammatory to an anti-inflammatory phenotype.Conversely,mesenchymal progenitors fromthe mouse aorta (mAo) support and contribute to the MΦ response under inflammatory conditions.We used cell lines with purported opposing immune-regulatory function, a bonemarrow derivedmesenchymal progenitor cell line (D1) and amouse aorta derived mesenchymal progenitor cell line (mAo). Their interaction and regulation of the MΦ cell response to the inflammatory mediator, lipopolysaccharide (LPS), was examined by coculture. As expected, D1 cells suppressed NO, TNF-𝛼, and IL-12p70 production but MΦ phagocytic activity remained unchanged. The mAo cells enhanced NO and TNF-𝛼 production in coculture and enhanced MΦ phagocytic activity. Using flow cytometry and PCR array, we then sought to identify sets of MSC-associated genes and markers that are expressed by these progenitor populations.We have determined that immune-supportive mesenchymal progenitors highly express chondrogenic and tenogenic transcription factors while immunosuppressive mesenchymal progenitors highly express adipogenic and osteogenic transcription factors. These data will be useful for the isolation, purification, and modification of mesenchymal progenitors to be used in the treatment of inflammatory diseases.

Related Pillar(s)

Study

Comments

Natalie Fernandez, Heather Renna, and Lauren McHugh are Molloy undergraduate students.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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